By Phyllis R. Brown, Eli Grushka
"Volume 35 examines well timed topics equivalent to functionality standards, detection modes, and ancillary innovations for optical detectors in capillary electrophoresis; and more."
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Extra info for Advances in Chromatography, Volume 35
Conventional light sources are never true point sources. Only a small fraction of the light output can be effectively coupled into these small areas. , photon-counting statistics). The short response time t effectively decreases the number of photons counted per measurement cycle. The result is that one roughly loses a factor of 200 from a shorter path length (50 µm vs. 05 mm2 vs. 2 s vs. 2 s) when compared with LC detectors. Even considering the smaller dilution factors inherent in the separation and the utilization of special optics, it is not surprising that concentration-sensitive detectors fare rather poorly in CE.
This is made possible by the good heat-dissipating capability of the capillary, which gives rise to sharp bands. The use of Schlieren optics for the whole-column detection of protein bands without mobilization has also been achieved . 12 Diagram of the cartridge holding the capillary, the probe beam, and the position sensor for Schlieren-optical detection. ) the very sharp boundaries in isoelectric focusing. However, its compatibility with the more popular forms of CE has not been demonstrated.
Lasers have the further advantage of allowing better focusing and thus can use the full internal diameter of the capillary as the absorption pathlength. However, lasers are inherently unstable in the output intensity. 1% in specially controlled ion lasers. Still, none of these noise levels can approach the 10-5 stability level of a good commercial lamp. What one gains in a longer pathlength and an extended shot-noise limit becomes irrelevant unless the intensity (flicker) noise can be controlled.